| abstract |
The present invention discloses a preparation method and product for high-efficiency expression of stem cells for skin beauty, wherein the preparation method comprises: separating stem cells from human tissue and culturing; constructing a recombinant expression vector and adding the recombinant expression vector to the culture of stem cells Suspension, use electrotransfection to obtain stem cells that can express the target protein, extract exosomes from the supernatant of stem cells, add excipients by weight and use liquid nitrogen for instant freeze-drying, and dry the obtained tablets in a vacuum environment. . Through the technical solution of the present invention, the recombinant expression vector can be transferred into stem cells, the target protein exosomes can be extracted, and the products for skin beauty can be prepared by freeze-drying technology, which effectively protects the active components of exosomes and can Long-term storage at room temperature and remain active. |