http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111434773-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-02 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 |
| filingDate | 2019-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111434773-B |
| titleOfInvention | Recombinant yeast for high-yield sandalwood oil and construction method and application thereof |
| abstract | The invention discloses a recombinant yeast for high-yield sandalwood oil and a construction method and application thereof, wherein the method comprises the following steps: 1) integrating genes such as acetoacetyl-CoA thiolase genes and the like from saccharomyces cerevisiae driven BY a strong promoter and CYP450 reductase genes driven BY a weak promoter into a genome of saccharomyces cerevisiae BY4742 serving as an initial strain in a homologous recombination manner; 2) replacing a promoter of a squalene synthase gene in the recombinant bacterium; knocking out genes such as galactose regulatory protein 80 gene and the like; 3) and (3) inserting a santalene synthase gene and a CYP450 monooxygenase gene driven by a strong promoter into the multicopy episomal plasmid to obtain an expression plasmid, and introducing the expression plasmid into the recombinant saccharomyces cerevisiae obtained in the step (2) to obtain the recombinant saccharomyces cerevisiae with high sandalwood oil yield. The invention overcomes the defect that the production of sandalwood oil is extracted from sandalwood, and the yield of the recombinant yeast strain per liter of sandalwood oil reaches 1 g. And the industrial production is satisfied. |
| priorityDate | 2019-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1230.