http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111424017-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-531 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-0008 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 |
filingDate | 2020-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-111424017-B |
titleOfInvention | A kind of exosome loaded with shRNA and its construction method and application |
abstract | The invention discloses an exosome loaded with shRNA and a construction method and application thereof. In the present invention, the recombinant expression vector 1 is obtained by cloning the shRNA sequence that can effectively silence the target gene into the expression vector; the shRNA sequence includes the target sequence, the stem-loop structure sequence and the complementary sequence of the target sequence connected in sequence; The gene of the protein A specifically bound by the stem-loop structure is connected with the gene of the exosome membrane protein to obtain a fusion gene sequence, and then the obtained fusion gene sequence is cloned into an expression vector to obtain a recombinant expression vector 2; finally, the recombinant expression vector 1 , 2 Simultaneously transfect cells, culture the transfected cells, and collect exosomes, that is, to obtain the shRNA-loaded exosomes. The method is simple and easy to operate, has stable process and good applicability. The obtained exosomes contain a large amount of shRNA, which can be used as an efficient drug delivery system. |
priorityDate | 2020-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 721.