http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111413172-A

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filingDate 2020-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a826a5902e9b7c2caf20bcaad979cca6
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publicationDate 2020-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111413172-A
titleOfInvention A kind of in situ enzymolysis-direct mass spectrometry analysis system and analysis method
abstract The invention belongs to the technical field of protein molecule detection, and in particular relates to an in-situ enzymatic hydrolysis-direct mass spectrometry analysis system and an analysis method. Wherein, the in-situ enzymolysis-direct mass spectrometry system includes a micro-enzymolysis cell, a mass spectrometer and an interface, etc. The enzymolysis cell includes a columnar part and a capillary part that are connected to each other; the columnar part is filled with protease and protein sample molecules , the protease is used for enzymolysis of protein sample molecules; the inner surface of the columnar part is coated with an adsorption material, and the adsorption material is used to capture the polypeptide fragments after enzymolysis; the end of the capillary part is processed by spray structure and mass spectrometry. The sample port is docked, and the spray structure is used to ionize the polypeptide fragments after enzymatic hydrolysis, so that the mass spectrometry device can directly perform mass spectrometry analysis. The invention can be used for rapid identification of protein molecules in foods such as milk and biological tissue media.
priorityDate 2020-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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