| abstract |
A method for researching the influence of mechanical force on bone includes such steps as preparing acidic simulative body liquid by ⑴, preparing in vitro bone sample by ⑵, fixing ⑶ the in vitro bone sample in mechanical apparatus, injecting said acidic simulative body liquid, applying mechanical force, analyzing by X-ray crystal diffraction technique, calculating the average crystal grain size of apatite crystal by Sheer formula, preparing bone cell sample by ①, preparing bone cell sample by ②, fixing it in mechanical apparatus by ③, injecting said cell nutrient liquid, applying mechanical force, extracting mRNA and protein, and real-time observing the influence of mechanical force on the fine structure of bone sample. |