http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111394384-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6897 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6897 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
| filingDate | 2020-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111394384-B |
| titleOfInvention | Biosensor for detecting S-adenosylmethionine and preparation method thereof |
| abstract | The invention discloses a biosensor for detecting S-adenosylmethionine and a preparation method thereof, belonging to the field of biochemical engineering. The biosensor takes MetJ from escherichia coli as a DNA binding domain, forms novel ATF with hER and an activation domain (VP16), simultaneously selects natural metbox with 4 conservative sequences from metA as a specific recognition sequence of MetJ, and is connected with a green fluorescent protein gene at the downstream of the sequence to construct the biosensor responding to SAM. Under the action of beta-estradiol inducer and intracellular SAM, ATF enters the cell nucleus and is combined with the specific recognition sequence met box of MetJ, thereby realizing downstream gene regulation. The biosensor can respond to the concentration of S-adenosylmethionine in a living cell and realize high-throughput detection through the output of strong and weak fluorescence intensity. |
| priorityDate | 2020-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.