http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111394382-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-01073 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-18 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2020-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111394382-B |
| titleOfInvention | Recombinant expression vector and recombinant bacterium of feruloyl esterase BpFae gene, and recombinant expression method |
| abstract | The invention discloses a recombinant expression vector and a recombinant bacterium of feruloyl esterase BpFae gene, and provides a method for recombinant expression of the enzyme under optimized expression conditions. The starting vector is pGEX-4T-1 to construct the gene expression vector containing the ferulic acid esterase BpFae, and when IPTG is used as an inducer, the optimal conditions are as follows: adopting an SOB culture medium; initial pH: 5.0; inoculation amount: 0.8% (v/v); the induction time is as follows: 4 h; IPTG concentration: 0.05 mM; induction temperature: 26 ℃; rotating speed of a shaking table: 240 rpm; induction time: 24h, the activity of the BpFae obtained in the way can reach 2.54U/mL at most; when lactose is used as an inducer, an LB culture medium is adopted; lactose concentration: 6 g/L; initial pH: 5.5; the induction time is as follows: 5 h; induction temperature: 23 ℃; rotating speed of a shaking table: 240 rpm; liquid loading amount: 50mL/250 mL; inoculation amount: 0.2% (v/v); induction time: the activity of the BpFae obtained in the way can reach 7.43U/mL at most after 32 h. The optimal induction expression condition of the invention can prepare the ferulic acid esterase BpFae more efficiently. |
| priorityDate | 2020-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 68.