http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111394307-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_41e2565f61e417bab0cca3794dc97209 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0634 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-078 |
| filingDate | 2020-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cd8fb3be6b7d95004f12ffa49c336f97 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_66b95e5c6a01f2a8ad2836cc0245825d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4082c729801a2b1650855cdb26d6ab44 |
| publicationDate | 2020-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111394307-A |
| titleOfInvention | A kind of method and application of separating and purifying exosomes from plasma |
| abstract | The invention relates to a method for separating and purifying exosomes from plasma. The steps are as follows: taking human plasma and centrifuging and then taking the supernatant; ultracentrifuging the centrifuged plasma; extracting the supernatant after centrifugation; Then invert; when there are no visible droplets in the tube, use filter paper to wipe the inner wall of the tube; use PBS to repeatedly blow the bottom of the tube, resuspend and inject PBS, mix well; filter the above resuspension; use PBS to filter the resuspension After the volume is replenished, add the ultracentrifuge tube; centrifuge, pour, and invert; when there are no visible droplets in the tube, wipe the inner wall of the tube with filter paper; add PBS or lysate to resuspend the pellet at the bottom of the tube to obtain exosomes. The method of the invention combines the ultracentrifugation and membrane filtration methods, and the operation is simple, the exosomes can be extracted from a small amount of plasma, and the extracted exosomes have a large content, which can be used for a series of biological experiments. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113403279-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112251394-A |
| priorityDate | 2020-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.