http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111394303-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-105 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-21 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0662 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-52 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-52 |
| filingDate | 2020-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111394303-B |
| titleOfInvention | Medium containing stem cell activator and method for culturing mesenchymal stem cells |
| abstract | The invention discloses a culture medium containing a stem cell activator and a culture method for mesenchymal stem cells. The culture method is as follows: 1) after culturing the mesenchymal stem cells with a cell culture medium to a degree of confluence of 80%, discarding the culture medium; adding Culture medium containing stem cell activator; cultivating; cleaning; 2) adding compound electrolyte solution, cultivating, collecting culture supernatant by half volume; 3) adding half volume compound electrolyte solution, cultivating, collecting culture supernatant by half volume; 4) Repeat step 3) 4-8 times; 5) Filter and concentrate the collected culture supernatant, purify and sterilize to obtain the stem cell factor group. The stem cell factor obtained by the method of the present invention is continuously stable, has a high concentration, does not contain bovine serum components, does not contain animal-derived components, and does not contain allergens. The method of the present invention is easy to operate and low in cost, and the stem cell factor stock solution can be prepared into different stem cells Factor preparation products are suitable for different indications and different administration methods. |
| priorityDate | 2020-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 242.