| abstract |
The invention discloses a method for detecting a novel coronavirus, comprising the following steps: preparing a qPCR reaction solution, an enzyme mixture, an amplification primer and a probe mixture, constructing a reaction system, amplifying, analyzing fluorescent signals, and judging whether the sample contains The target gene is present. The detection method of the present invention combines two genes with reverse transcription and qPCR operations, which can reduce missed detection caused by very little virus content in the specimen and false detection caused by other coronaviruses in the early stage of infection, and improve the speed and accuracy of detection. |