| abstract |
The invention discloses a nucleic acid visual detection kit for novel coronavirus SARS-CoV-2. The kit of the invention includes primer set, RT-LAMP reaction solution, positive control, negative control, DEPC water and other components. The primer set used to detect SARS-CoV-2 includes 6 primers of outer primers F3 and B3, inner primers FIP and BIP, and loop primers LF and LB, whose sequences are shown in SEQ ID NO: 1 to NO: 6. The above primer sets and kits can accurately detect the S gene of SARS-CoV-2 and have no cross-reaction with other coronaviruses and other pneumonia-causing viruses. Isothermal amplification at 63°C can detect SARS-CoV-2 in as fast as 15 minutes. The detection sensitivity reached 1.6fg/μL. The invention has the advantages of simplicity, rapidity, sensitivity and specificity, and can directly determine the result by observing the color change of the reaction tube with the naked eye under natural light without using expensive instruments, which can realize the rapid and accurate detection of SARS-CoV-2, and is suitable for It is used for on-site testing and clinical testing in primary hospitals. |