http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111378022-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43595 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-64 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 |
| filingDate | 2018-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111378022-B |
| titleOfInvention | Fluorescent protein and preparation method and application thereof |
| abstract | The invention provides a fluorescent protein, a preparation method and application thereof, relates to the technical field of biology, screens out a novel red fluorescent protein with large Stokes displacement, can be effectively excited by single-color light to carry out multicolor imaging in single-photon and multi-photon microscopic imaging together with green fluorescent protein, and has good monomer property and greatly improved light stability. Meanwhile, the luciferase can be used as a donor to be combined with receptor luciferase, and a novel high-sensitivity bioluminescence resonance energy transfer system is formed in an organism. When the intracellular intermolecular interaction is detected, the dynamic range of the bioluminescence resonance energy transfer system is improved by 2.27 times compared with the traditional bioluminescence resonance energy transfer system. By applying the protein provided by the invention, the efficiency of a BRET system is improved, and the dynamic range of the interaction between detection molecules by using a bioluminescence resonance energy transfer system is improved. |
| priorityDate | 2018-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 197.