| abstract |
The present invention provides an analytical method for the determination of lubiprostone test substance related substances. The method adopts reversed-phase high performance liquid chromatography to separate and determine the lubiprostone compound, the compound of formula I, the compound of formula II and the compound of formula III. Compounds, Compounds of Formula IV and Compounds of Formula V. The solvent of this method does not interfere with the detection of impurities, the specificity of the method is good, and the detection method is simple, high in sensitivity, good in repeatability and good in accuracy. Controllability of ketone quality. |