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filingDate 2020-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2021-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111349707-B
titleOfInvention A method for detecting copy number variation of ZNF280BY gene in sheep and its application
abstract The invention discloses a method for detecting the copy number variation of ZNF280BY gene in sheep and its application. The invention uses the whole genome DNA of the ram to be tested as a template, and uses male-specific qPCR primer pairs P1 and P2 to amplify the ZNF280BY gene of the ram respectively. and a partial fragment of the DDX3Y gene, wherein the multi-copy ZNF280BY gene is used as a detection gene, and the single-copy DDX3Y gene is used as a reference gene, and the copy number of the ram ZNF280BY gene is calculated according to the quantitative result, and the present invention can detect the copy for early identification of the ram fecundity. The number of variable molecular markers can be used to speed up the breeding process of rams through molecular marker-assisted selection. The method of the invention is simple, fast and convenient for popularization and application.
priorityDate 2020-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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