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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90
filingDate 2020-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2022-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2022-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111349647-B
titleOfInvention CRISPR/Cas 9-mediated microbial secondary metabolite enhanced initiation system for additional endogenous RBS
abstract The invention discloses a CRISPR/Cas 9-mediated microorganism secondary metabolite enhanced start system with an additional endogenous RBS, and belongs to the technical field of genetic engineering. The invention realizes the combination of an endogenous ribosome binding site and an exogenous promoter based on a CRISPR/Cas9 gene targeting system, and constructs a microorganism secondary metabolite enhanced start system with an additional lRBS; meanwhile, the yield of the positive mutant strain is improved by combining the interspecific combined transfer of the spores, so that the yield of the secondary metabolite of the microorganism is obviously improved.
priorityDate 2020-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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