| abstract |
The invention relates to a duck circovirus tissue inactivated vaccine and a preparation method of a yolk antibody thereof, wherein the antigen liquid of the vaccine is prepared by grinding, repeatedly freezing and thawing, centrifuging and filtering a grinding liquid containing duck circovirus liver tissue and sterile water according to the mass ratio of 1:3-6, and pollen pini polysaccharide with the concentration of 5-40mg/mL is added into the antigen liquid as an immunopotentiator and is emulsified with a white oil adjuvant; the yolk antibody is obtained by immunizing laying hens with the vaccine and then extracting and purifying the yolk of the hyperimmune egg; the provided duck circovirus tissue inactivated vaccine has the advantages of simple preparation process, low cost, good effect, stable dosage form, easy storage, huge development and utilization potential in the duck breeding industry and wide application prospect. The yolk antibody prepared by the invention has good safety, does not have any local or systemic adverse reaction caused by the yolk antibody, can effectively prevent and treat the infection of the duck circovirus, and has good commercial development prospect. |