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filingDate 2020-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2020-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111337485-A
titleOfInvention A colorimetric detection method of hexavalent chromium based on silver nanocluster nanozyme
abstract The invention discloses a hexavalent chromium colorimetric detection method based on silver nano-cluster nano-enzyme. The method includes the following steps: (1) uniformly mixing PEI and silver nitrate in deionized water, adjusting pH with acetic acid, adding ascorbic acid and bathing in water until the reaction is complete, to obtain a light yellow PEI-Ag NCs solution; (2) mixing PEI-Ag The NCs solution, acetic acid-sodium acetate, and TMB were mixed evenly, and the hexavalent chromium aqueous solution was added, and the reaction was complete at 20-75 °C; the obtained mixed solution was measured for ultraviolet absorption spectrum, and the absorbance at the wavelength of 652 nm was recorded and the hexavalent chromium concentration was drawn. Absorbance standard working curve; (3) Repeat the previous step with the solution to be tested, and compare the measured absorbance with the standard working curve to obtain the hexavalent chromium concentration. The invention utilizes environmentally friendly and low-cost nano-enzymes, can quickly and conveniently determine Cr 6+ in environmental water samples, has lower secondary pollution, and is suitable for on-site water sample detection.
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