http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111334611-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-166 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-682 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
filingDate | 2020-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-111334611-B |
titleOfInvention | Kit for detecting novel coronavirus (2019-nCoV) based on double amplification technology and application thereof |
abstract | The invention discloses a kit for detecting a novel coronavirus (2019-nCoV) based on a double amplification technology and application thereof. The collected sample is cracked by cell lysate to release pathogen nucleic acid, and amplification of pathogen nucleic acid fragments is realized through reverse transcription and transcription processes. Adding the amplified RNA product into a micropore coated with a coating probe, and simultaneously adding a specific probe and an amplification probe, wherein the coating probe can be combined with one end of a specific probe CES to fix the RNA of the amplified product; one end of a specific probe LES is combined to the RNA product, and the other end is combined with an amplification probe to realize signal amplification. The invention does not need RNA extraction, is not easy to pollute in detection, has high sensitivity and strong specificity, can quickly and efficiently detect the novel coronavirus (2019-nCoV), and is greatly helpful for spreading and preventing epidemic situations. |
priorityDate | 2020-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 95.