http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111334545-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a5636c57aabc1afed1025550d4c1ddbd |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-70575 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 2020-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6d15363efd957d263b2fa5850d8ddf12 |
| publicationDate | 2020-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111334545-A |
| titleOfInvention | Preparation process of high-purity recombinant TRAIL |
| abstract | The invention discloses a preparation process of high-purity recombinant TRAIL, belonging to the field of gene recombination. Comprises five steps of thallus inoculation, fermentation culture, feeding culture, crushing collection and chromatographic separation. According to the invention, the promoter structure of gene recombination is researched, and acid-hydrolyzed casein is selected as an induced nutrient component, so that the yield of the recombined TRAIL is increased; when ferrous ions are used as chelate ions, not only can a better chromatographic separation effect be achieved, but also the growth of escherichia coli can not be negatively affected; the pH value of the nutrient components is reasonably controlled by researching a feeding mode, so that the ferrous ions are prevented from being converted into toxic substances to influence the biological activity of the recombinant TRAIL; the separation rate of the recombinant TRAIL is increased by further optimizing thallus crushing and TRAIL collection and selecting coarse silica gel as an adsorption medium. The yield and the purity of the TRAIL are improved. |
| priorityDate | 2020-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 58.