http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111334520-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-91171 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-924 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-91017 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 |
| filingDate | 2020-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111334520-B |
| titleOfInvention | Preparation method of interpolymer enzyme compound, interpolymer enzyme compound and application |
| abstract | The invention provides a preparation method of an interpolymer enzyme compound, the interpolymer enzyme compound and application, belonging to the technical field of protein engineering technology and biomarker diagnosis, comprising the following steps: fusing SAM gene with SpT gene and SnT gene, and inserting into vector to obtain recombinant plasmid; after the SAHH gene and the SnC gene are fused, inserting the SAHH gene and the SnC gene into a vector to obtain a recombinant plasmid; after the HMT gene and the SpC gene are fused, inserting the fused HMT gene and the SpC gene into a vector to obtain a recombinant plasmid; and (3) respectively or jointly transforming the three obtained recombinant plasmids into competent cells to obtain engineering bacteria, and performing induced expression on the engineering bacteria to obtain a co-polymer enzyme compound. The preparation method of the invention improves the enzyme activity of the copolymer enzyme compound, enhances the stability, can improve the production efficiency and reduce the addition of the key enzyme when preparing the Hcy detection reagent, thereby reducing the cost. |
| priorityDate | 2020-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1068.