http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111333618-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09K2211-1007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09K2211-1029 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09K2211-1044 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6432 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D473-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D519-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D401-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6458 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6456 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09K11-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K49-0021 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D401-14 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D473-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C09K11-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K49-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D401-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D401-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D519-00 |
| filingDate | 2018-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111333618-B |
| titleOfInvention | 488nm excited wash-free SNAP-tag probe and preparation method thereof |
| abstract | The invention provides a 488nm excited wash-free SNAP-tag probe and a preparation method thereof, the wash-free SNAP-tag probe is a series of wash-free SNAP-tag probes which are designed and synthesized by introducing a spiral ring rigid structure into a 4, 5-position disubstituted naphthalimide dye and can be used for 488nm excitation, and the structural formula is shown as (1). The introduction of the rigid structure not only limits intramolecular torsion, improves the stability and brightness of the fluorescent molecule, but also increases the planarity of the probe molecule. This results in the SNAP-tag probe molecule forming a strong pi-pi effect in water, resulting in quenching of fluorescence. After the probe molecule is combined with SNAP-tag, the fluorescence of the probe molecule is released, and the maximum fluorescence can be enhanced by 28 times. The series of probes can realize the specific marking of SNAP-tag in living cells, and achieve the purpose of washing-free fluorescence imaging. In addition, due to the improvement of stability and brightness, the series of probes can also be used in the super-resolution fluorescence imaging fields of SIM (structured light microscope), STED (stimulated radiation loss) and the like. |
| priorityDate | 2018-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 65.