http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111321177-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_72206ca96c18ac1fe3f5b21bb02a5bb1 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-001 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-00 |
| filingDate | 2020-03-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_118909717102bae059153890541c04ea http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a5838a34d91f912e1565312f4c2b7ab http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bcfdf67f3ab7666c779a562a787e914f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7d0b889fb10e8b511ccb14ecad6aa179 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_73815b2b8f487a768af2a318ac3ed9aa |
| publicationDate | 2020-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111321177-A |
| titleOfInvention | A kind of method for enzymatic synthesis of cinacalcet intermediate (R)-1-(1-naphthyl)ethylamine |
| abstract | The invention discloses a method for enzymatically synthesizing cinacalcet intermediate (R)-1-(1-naphthyl)ethylamine. The gene sequence and amino acid sequence of the used D-amino acid dehydrogenase are as shown in SEQ ID NO. As shown in 1 and NO.2, it can be prepared by constructing Escherichia coli genetic engineering bacteria and fermenting culture in large quantities; in the potassium phosphate buffer of pH6.5～8.5, add 50～300mmol/L 1-naphthoethyl ketone concentration respectively, 100～ 600mmol/L amino donor, 0.1-0.5g/L coenzyme, 5%-10% (V/V) co-solvent, 1-3g/L coenzyme cycle enzyme, 0.5-2g/LD-amino acid dehydrogenase, at 30 The reaction is carried out at ~40° C. for 24 to 48 hours to generate (R)‑1‑(1‑naphthyl)ethylamine, the final reaction conversion rate is greater than 95%, the product yield is greater than 88%, and the product chiral purity reaches 99% ee. The invention has mild reaction conditions, small amount of organic solvent, greener reaction system and high conversion rate, greatly improves the preparation efficiency of (R)-1-(1-naphthyl)ethylamine, and has good industrial application prospects. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112387262-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112387262-B |
| priorityDate | 2020-03-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 177.