http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111307990-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-067 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-8637 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-8679 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-86 |
| filingDate | 2020-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111307990-B |
| titleOfInvention | Method for simultaneously determining content of cystine and methionine in artificial tiger bone powder |
| abstract | The invention relates to a method for simultaneously determining the content of cystine and methionine in artificial tiger bone powder, which comprises the following steps: (1) adding performic acid solution into the artificial tiger bone powder sample for oxidation, adding hydrobromic acid to terminate the oxidation, evaporating to dryness, adding hydrochloric acid for hydrolysis, evaporating to dryness, dissolving with derivative buffer solution, and deriving to obtain a test sample solution; (2) dissolving cysteic acid and methionine sulfone reference substances respectively with a derivative buffer solution to obtain a mixed reference substance solution through derivation; (3) respectively carrying out chromatographic detection on the test solution and the reference solution, wherein cyano-bonded silica gel is used as a filler in a chromatographic column; column temperature 20 ℃; mobile phase: a is sodium acetate buffer solution; b is methanol-acetonitrile-water, and gradient elution is carried out; (4) and calculating the contents of the cysteic acid and the methionine sulfone in the artificial tiger bone powder according to the detection result, and further calculating the contents of the cystine and the methionine sulfone. The detection method is accurate, reliable, simple, convenient and quick. |
| priorityDate | 2020-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 58.