http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111304298-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_653c418e41edbb4e1ce8bc5e53e70cec |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 |
| filingDate | 2020-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a616f0763ff34c4808bd0cffcce02a42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6bbea0b12b9895a70be4526db4c52466 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_720510ba6dc4e8a1c7fe6616965f17d0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c81b783b5fe9704b5388c81d2ebaf34e |
| publicationDate | 2020-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111304298-A |
| titleOfInvention | Caspase biosensor and application, detection method of caspase activity |
| abstract | The invention discloses a caspase biosensor and its application, a detection method for caspase activity, and a caspase biosensor, comprising magnetic beads, detection probes, circular templates, DNA polymerase, and secondary primers ; The surface of the magnetic beads is coated with streptavidin; the detection probe includes a linked peptide substrate domain and a DNA primer domain, the peptide substrate domain is an amino acid sequence, and the DNA primer domain is a single-stranded DNA sequence; The substrate domain contains a specific site, which can be recognized and cleaved by caspases. The specific site is n amino acids away from the linking site. The linking site is the connection between the peptide substrate domain and the DNA primer domain. where, the peptide substrate domain is linked with biotin, the specific site is located between the biotin and the linking site, and n is a natural number less than 10; the DNA primer domain can cooperate with circular template, DNA polymerase, and secondary primers Branched rolling circle amplification reaction. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111979295-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111979295-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113106147-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113106147-A |
| priorityDate | 2020-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 87.