http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111304194-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_53505a2db1019521f0e063b41802da02 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-186 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-3231 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-163 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2019-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_17bde9c764fb3db3c03c2c5e542f316c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_708427939345bf8aac91e3f912440306 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb2f7d1e08a79d90418ffb6015218784 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4042759a5a4f2a3c6e4e1ad8f841551b |
| publicationDate | 2020-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111304194-A |
| titleOfInvention | A nucleic acid amplification blocker for detecting low-abundance mutant sequences and its application |
| abstract | The invention discloses a design principle of a nucleic acid amplification blocker for detecting low-abundance mutant sequences, and relates to the field of mutant gene detection. The nucleic acid amplification blocker is an oligonucleotide modified by locked nucleic acid (LNA). , the paired region of the nucleic acid amplification blocking agent is located between the amplified sequences, and is completely matched with the wild-type gene sequence, and has at least one mismatch with the mutant sequence. The invention also discloses the application of the nucleic acid amplification blocking agent in detecting low-abundance mutant sequences. The present invention uses the LNA-modified nucleic acid amplification blocker to have a huge difference in affinity with the mutant nucleic acid sequence/wild-type nucleic acid sequence, so as to achieve the purpose of amplifying/enriching the mutant sequence in the sample with high selectivity. The reagent has the advantages of high affinity, flexible base modification position, good thermal stability, low price, etc. The detection effect of the invention on deletion mutation and insertion mutation is more significant. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022099938-A1 |
| priorityDate | 2018-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.