| abstract |
The present application belongs to the technical field of nucleotide production, and discloses a preparation method of taste-presenting disodium nucleotides. Inosine or guanosine and phosphoric acid donor are mixed with water to form a suspension solution, phosphorylase is added to carry out an enzymatic reaction, and the enzymatic reaction liquid is collected to cool down for phosphorus separation, decolorization, and cooling to crystallize to obtain high-purity taste-flavored disodium nucleotides. . The preparation method of the invention has strong biocatalysis specificity, simple operation, and can quickly obtain disodium nucleotides with taste. The taste-presenting nucleotide disodium products 5'-inosinate disodium and 5'-guanylate disodium products prepared by the preparation method of the present invention have high purity, and the purity can reach more than 97.5%, meeting the food-grade requirements. The preparation method of the invention has simple and easy-to-obtain raw materials, mild reaction conditions and low energy consumption, can be rapidly scaled up and applied to industrial production, and simultaneously realizes the concept of green production. |