| abstract |
The invention relates to a 3D printing technology-based method for preparing a bionic capillary vessel network with specific growth, which comprises the steps of preparing hydrogel containing three-dimensional channels and growth factors by utilizing the 3D printing technology, inoculating endothelial cells into the channels for culturing for a period of time, supplementing a growth factor solution to a specific part, and continuing culturing for a period of time to obtain the bionic capillary vessel network with specific growth, wherein the three-dimensional channels are human artery or vein vessel network-shaped structures and are composed of a plurality of cylindrical channels, the center distance between every two adjacent cylindrical channels is less than or equal to 5mm, and the hydrogel is hydrogel which can be invaded by the endothelial cells and differentiated cells thereof. The method for manufacturing the bionic capillary vessel network with specific growth based on the 3D printing technology is simple and easy to implement, low in cost and environment-friendly, and can effectively control the density degree of the blood vessel branches at specific positions according to needs. |