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filingDate 2020-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e5734fb37fdccb0d693c2394af924f6a
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publicationDate 2020-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111273030-A
titleOfInvention A direct competition ELISA kit for quantitative detection of recombinant human epidermal growth factor and its application
abstract The invention discloses a direct competition ELISA kit for quantitative detection of recombinant human epidermal growth factor and its application, including a microporous enzyme label plate coated with a recombinant human epidermal growth factor polyclonal antibody, a recombinant human epidermal growth factor standard, and an enzyme marker Recombinant human epidermal growth factor antigen, chromogenic solutions A and B, 20X concentrated wash solution, sample diluent and stop solution. Through antigen-antibody specific reaction, based on enzyme immune technology, a direct competitive ELISA method was established and developed to quantitatively detect recombinant human epidermal growth factor in fermentation broth, semi-finished products, finished products, etc. and clinical tissue cell samples. Methodology and commercialization The kit can be used for the development of corresponding products and the specific detection of clinical samples; the minimum detection limit of the method of the invention can reach 2.0ng/ml, the intra-batch difference is less than 5%, and the inter-batch difference is less than 10%; the recovery rate is 90% % to 110%, indicating that the kit has important commercial development value for manufacturers to control product quality and detect clinical specimens.
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