http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111269872-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0601 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-07 |
| filingDate | 2020-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111269872-B |
| titleOfInvention | Method for separating scylla paramamosain tissue exosomes |
| abstract | The invention relates to a method for separating exosome of scylla paramamosain tissue, which mainly comprises S1) tissue extraction; s2) tissue digestion; s3) low-speed centrifugation; s4) low speed centrifugation is performed again; s5) high-speed centrifugation; s6) centrifuging at high speed again; s7) resuspending the pellet; s8) sucrose density gradient centrifugation; s9) ultracentrifugation; s10) ultracentrifugation again; s11) dialyzing by a dialysis bag; s12) filtering with a filter membrane; s13) ultracentrifugation; s14) resuspending the pellet to obtain the isolated exosome product extracted. The method for extracting the scylla paramamosain tissue exosomes combines the methods of differential centrifugation, sucrose density gradient centrifugation and dialysis filtration, is stable and effective, has high purity and simple operation, and does not need special equipment. The obtained exosome has high content and high purity. |
| priorityDate | 2020-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 61.