| abstract |
The present invention provides a preparation method of an HPV16 type E7 protein sandwich immunodetection kit, comprising: (1) preparing a sandwich structure top layer solution; (2) preparing a sandwich structure substrate solution, and the kit prepared by the above method also includes a human body Cervical exfoliated cell lysate, during use, lyses human cervical exfoliated cells, immobilizes HPV16 E7 protein through the sandwich structure substrate solution, and then adds the top layer solution of the sandwich structure to form a sandwich structure. When testing, the sandwich structure is pulled. The light signal is generated under the MAN laser to realize the detection of HPV16 type E7 protein. This method makes up for the defects of complex detection procedures and low detection efficiency of high-risk human papillomavirus in the prior art. Precancerous prediction provides a simple, rapid, sensitive and specific detection method. |