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filingDate 2019-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2022-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2022-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111239267-B
titleOfInvention A method for detecting short-chain fatty acids in serum and lymphoid tissue based on GC-MS
abstract The invention discloses a method for detecting short-chain fatty acids in serum and lymphoid tissue based on GC-MS, comprising the following steps: preprocessing lymphoid tissue to obtain a lymphatic extract; adding an internal standard substance to the serum or lymphatic extract, And adding pentafluorobromobenzine to carry out derivatization reaction; after the derivatized solution is extracted, acetic acid, propionic acid and butyric acid in the extract are detected by GC-MS. The invention establishes a gas chromatography-mass spectrometry rapid determination method for detecting three kinds of short-chain fatty acids in serum and lymphoid tissue through a derivatization method. Pre-process and derivatize different biological samples, and add internal standard for correction to achieve accurate quantification.
priorityDate 2019-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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