http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111239094-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6402 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-38 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-38 |
| filingDate | 2020-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111239094-B |
| titleOfInvention | A kind of sensitive detection method of alkaline phosphatase |
| abstract | The invention discloses a sensitive detection method of alkaline phosphatase. The technical scheme is that the specificity of ALP for phosphate groups is used to react with AAPi to generate ascorbic acid AA, and then the reaction of AA and DES generates strong fluorescence, so as to realize the detection of Determination of ALP activity. The invention adopts 4,4'-diazide stilbene-2,2'-disulfonic acid disodium tetrahydrate (DES) as a fluorescent probe, and has the advantages of low cost, simple detection and high sensitivity. The method of the invention has the advantages of less false signal, high sensitivity, good selectivity, rapid detection and the like. Experiments show that the detection linear range of the invention is 5-40mU/mL, the correlation coefficient is 0.998, and the detection limit is 1.46mU/mL, which has high selectivity and anti-interference ability, and lays an important foundation for later application in clinical detection. |
| priorityDate | 2020-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 59.