| abstract |
The invention provides a CRISPR method for rapid detection of novel coronavirus, a method for rapid detection of novel coronavirus based on CRISPR-Cas13 system, by preparing each component of LwaCas13a protein, EiCsm6 protein, Csm6 activator, LwaCas13a-crRNA, RNase inhibitor reagent, reaction buffer, LF-Reporter detection kit, mix the liquid to be detected, the entire mixing operation is carried out at 0 °C, and then incubate in a constant temperature water bath for a certain period of time, add RNase-free DEPC aqueous solution to make up 50 microliters, using disposable nucleic acid test strips to test negative or positive. Compared with conventional methods, it greatly improves the detection efficiency of the new coronavirus. The detection time only takes half an hour, which is easy to use and portable. And the goal of rapid detection of the new coronavirus, and greatly reduce the cost. |