http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111235258-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_71de0b426be6a23c375c5536cbe2ad60 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-106 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6883 |
| filingDate | 2018-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d85bf79e375a769a45714e74ea254fff http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_809dfb7b687b1777e543ca7045e01784 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0651df14276311c8f264a10e1a75d52c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1566eb41c769d26bee960ccf79042d7 |
| publicationDate | 2020-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111235258-A |
| titleOfInvention | Method for distinguishing lacidipine personalized medicine by using primer composition to perform mass spectrometry |
| abstract | The invention provides a method for simultaneously detecting a plurality of sites related to metabolism of lacidipine of antihypertensive drugs by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) by utilizing extension primers with different Single Nucleotide Polymorphism (SNP) sites and different molecular weights, and finally obtaining the method for distinguishing the individualized administration of lacidipine by mass spectrometry through a primer composition, which comprises the following steps: respectively designing a multiplex amplification primer and an extension primer according to 6 target SNP sites to be detected; preparing a multiple amplification primer reaction system and an extension primer reaction system; in a reaction system, a plurality of sets of primers are used for simultaneously and respectively carrying out amplification and single base extension reaction on 6 target SNP sites; and (3) performing flight time mass spectrometry on the product obtained after the single base extension reaction, identifying the genotype of SNP (single nucleotide polymorphism) related to different drug metabolism according to the products of the extension primers with different molecular weights represented by mass spectrum peaks, and guiding the administration of the antihypertensive drug lacidipine. The invention can simultaneously detect 6 SNP sites related to metabolism of lacidipine, which is a antihypertensive drug, and has the advantages of low cost, no need of synthesizing probes, short time consumption, simple and convenient result analysis and extremely wide application field. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112831557-A |
| priorityDate | 2018-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 95.