http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111220720-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-8679 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
filingDate | 2019-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-111220720-B |
titleOfInvention | Purity detection method of trypsin and its zymogen |
abstract | The invention discloses a method for detecting the purity of trypsin and its zymogen. The purity detection method includes the following steps: the test solution is detected by size exclusion chromatography combined with ultraviolet detection method; the eluent in the size exclusion chromatography includes mobile phase A and mobile phase B; mobile phase A is an aqueous solution of trifluoroacetic acid containing halide ions, and the pH of mobile phase A is 1.7 to 2.1; in mobile phase A, the concentration of halide ions is 3 to 8 mM, excluding 8 mM; mobile phase B is a nitrile solvent; mobile phase The volume ratio of A to mobile phase B is 35:65 to 65:35. The purity detection method has the characteristics of good separation, simple and rapid, strong specificity, high accuracy, good repeatability and good linearity in the range of relative molecular mass from 3.5kD to 48kD. It can be used for process quality control and final product quality evaluation in drug synthesis process and preparation process. |
priorityDate | 2019-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 271.