http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111218430-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-6409 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0038 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-6409 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 |
filingDate | 2018-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-111218430-B |
titleOfInvention | NADH analogue dependent cytochrome P450reductase and application thereof |
abstract | The invention discloses an NADH analogue dependent cytochrome P450reductase and application thereof. NADH analogue is used as a cofactor and reducing power to catalyze electron transfer and reduce cytochrome P450 to complete catalytic cycle. The enzyme is fused and expressed with different cytochrome P450 enzymes to construct NADH analogue dependent, heterozygous and self-sufficient cytochrome P450 enzyme, the obtained NADH analogue dependent cytochrome P450 enzyme can be coupled with oxidoreductase for regenerating NADH analogue, and NAD analogue is utilized to catalyze corresponding substrate of different families of cytochrome P450 to be converted into product. The NADH analogue-dependent cytochrome P450reductase can be used for constructing a bioorthogonal metabolic pathway independent of a natural cofactor NAD (P) H, and decoupling energy consumption catalyzed by P450 enzyme from endogenous energy metabolism. |
priorityDate | 2018-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 247.