| abstract |
The invention relates to a preparation method of a composite scaffold for promoting skin regeneration, which comprises the following steps: (1) dissolving silk fibroin in a calcium chloride ternary dissolution system to form a silk fibroin solution; (2) dissolving chitosan in acetic acid to form a chitosan solution; (3) dissolving lithium chloride in an acetic acid solution to form a lithium chloride solution; (4) mixing the silk fibroin solution with the chitosan solution, and then adding the mixture into the lithium chloride solution to be stirred and incubated; (5) and after the incubation is finished, neutralizing the reaction solution, freezing and drying to obtain the composite scaffold for promoting skin regeneration. The composite scaffold has the advantages of interconnected pores, high porosity, high swelling ratio, controlled degradation, no cytotoxicity and the like, can prevent bacterial infection, reduce the replacement times, promote the regeneration of epithelialization and dermis, participate in the regulation and control of the whole skin repair process, is a promising sterile wound auxiliary material, and provides a new idea for developing novel wound dressings. |