http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111206034-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-02 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0625 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate | 2020-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111206034-B |
| titleOfInvention | New application of pig GADD45a gene and construction and application of high-expression cell line |
| abstract | The invention discloses a new application of a pig GADD45a gene and construction and application of a high-expression cell line. Designing and synthesizing a GADD45a full-length amplification primer containing a restriction enzyme cutting site according to a pig GADD45a gene full-length sequence and a vector pLEX-MCS; amplifying a GADD45a gene full-length sequence from a piglet adipose tissue, recovering and carrying out enzyme digestion, connecting the sequence with the enzyme digested pLEX-MCS, constructing a pLEX-MCS-GADD45a lentiviral plasmid, carrying out monoclonal sequencing identification and verification, and then co-transfecting the pLEX-MCS-GADD45a lentiviral plasmid, pSPAX.2 and pMD2.G into 293T cells by adopting a liposome transfection method to obtain a recombinant lentivirus; transfecting a pig IPEC-J2 cell by using the recombinant lentivirus obtained in the step 2) to obtain an IPEC-J2 cell line of a high-expression pig GADD45a gene. The new application of the pig GADD45a gene is used for regulating and controlling nutrition absorption and metabolism. The invention develops a new function of the pig GADD45a gene and a new method for researching the same in intestinal epithelial cells. |
| priorityDate | 2020-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 198.