http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111187863-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_052a1dd8f090f6f486c1338e84cd0d6d |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
| filingDate | 2020-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15bbeca8d867063106b2210b6c182dd6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b57d601d853efd9eabe8fd46bb23cd7d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c531e15e6781a7f6ccb2fe8e6792ab11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4bccb9021f3897209cd559b08ebdc54c |
| publicationDate | 2020-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111187863-A |
| titleOfInvention | A kit and detection method for double-enzymatic isothermal amplification detection of COVID-19 |
| abstract | The invention discloses a kit and a detection method for detecting COVID-19 by double-enzyme constant temperature amplification. The kit includes Forward Primer, Reverse Primer, MNAzymePart A, MNAzymePart B and Sub2‑FB. The detection method of the present invention is based on the combination of MNAzyme on the basis of the RPA recombinase constant temperature amplification technology, and adopts specific non-uniform amplification to generate enough linear DNA to make the MNAzyme continue to emit light; the MNAzyme working temperature is very wide, room temperature ( Fluorescence can be detected under the condition of 37°C), which is consistent with the working temperature of RPA amplification (37°C); MNAzyme has high luminescence specificity and catalytic specificity; compared with the traditional RPA constant temperature amplification technology, the method of the present invention There is no need to synthesize complex probe sequences, and multiple MNAzymes (2-3) can be set to obtain strong output fluorescent signals, avoiding the situation that the target nucleic acid signal cannot be detected due to weak signals; this method is simple, fast, and can be combined with a variety of When the instrument is used together, amplification can be achieved at both 42°C and 37°C without noise. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111549182-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111549182-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11440014-B2 |
| priorityDate | 2020-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.