| abstract |
The invention discloses a method for detecting Morganella by direct PCR of bacterial liquid, comprising the following steps: extracting bacteria from a sample to be tested and preparing a bacterial suspension, using the bacterial suspension as a template, constructing a PCR reaction system for PCR amplification ; The PCR amplification product is detected by gel electrophoresis. If the 857bp band is amplified, it indicates that the sample to be tested contains Morganella; if the 857bp band cannot be amplified, it indicates that the sample to be tested does not contain Morganella. Morganella. The present invention directly uses the treated bacterial suspension as a template to perform PCR detection on Morganella, saves complicated DNA extraction steps, simplifies PCR detection steps, saves manpower and material resources, and greatly reduces detection costs. |