http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111172236-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-916 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-42 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-42 |
| filingDate | 2020-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111172236-B |
| titleOfInvention | Alkaline phosphatase determination method based on path selector with alkaline phosphatase as switch, kit and application of kit |
| abstract | The invention belongs to the technical field of biological analysis and detection, and particularly relates to an alkaline phosphatase determination method based on a path selector with alkaline phosphatase as a switch, a kit and application thereof. The invention designs double-stranded deoxyribonucleic acid with two orbitals. The track 1 takes 5 ' -FAM and 3 ' -BHQ-1 as fluorescent probes, and the tail end of the 5 ' -FAM is provided with a 2-nt protruding structure. Orbital 2 is a ssDNA with 5 '-PO 4, the majority of the sequence being complementary to the probe, with 0-10nt overhang at the end of 5' -PO 4. In the absence of alkaline phosphatase, lambda exo selects for binding to the 5' -PO4 end and digests the complementary strand, resulting in a low fluorescence signal. When alkaline phosphatase is present in the sample, 5 '-PO 4 in orbital 2 is converted to the 5' -hydroxyl group; lambda exo selects binding to the 5' -FAM end and digests the probe to leave FAM and BHQ-1 and emit a strong fluorescent signal. |
| priorityDate | 2020-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.