http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111172090-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-14 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-15 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
filingDate | 2020-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-111172090-B |
titleOfInvention | Method for promoting corynebacterium crenatum to synthesize L-arginine by using ion transport protein |
abstract | The invention discloses a method for promoting corynebacterium crenatum to synthesize L-arginine by using ion transport protein, and particularly discloses a method for synthesizing L-arginine by over-expressing monovalent cation/H + A method for improving the yield of L-arginine by reverse transporter Mrp1A and cationic transporter ATP enzyme CTAP1 belongs to the technical field of biological engineering. The invention successfully realizes the knockout and over-expression of mrp1 and ctap1 genes by using intracellular Na + ]And [ K ] + ]The regulation of ion transport proteins in terms of ion concentration, pH homeostasis and osmotic pressure is well defined. A batch fermentation strategy of a 5L fermentation tank is adopted, fermentation conditions are optimized, and finally the recombinant corynebacterium crenatum 5-5(mrp1ctap1) is fermented for 96h, the L-arginine yield of the recombinant bacteria reaches 65.3g/L, the yield is 0.395g/g, and the yield is improved by 39.1% compared with that of corynebacterium crenatum SYPA 5-5. |
priorityDate | 2020-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 492.