http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111171229-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08F220-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J13-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08F8-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08F4-04 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J13-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08F222-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08F4-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08F220-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08F220-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08F8-48 |
filingDate | 2020-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-111171229-B |
titleOfInvention | Preparation method of nucleic acid immobilized microspheres |
abstract | The invention provides a preparation method of nucleic acid immobilized microspheres, which can effectively immobilize nucleic acid molecules. The method comprises the following steps of dissolving water-soluble functional acrylamide azide monomer, acrylamide monomer, N-methylene-bisacrylamide and polyvinylpyrrolidone in a mixed solvent of isopropanol and water; adding a free radical initiator, heating to 70-90 ℃ from room temperature, and carrying out polymerization reaction to obtain the target functional polymer microspheres; the polymer microsphere can effectively realize the immobilization of nucleic acid on the microsphere through the reaction of the reactive azide group and the alkynyl nucleic acid molecule. |
priorityDate | 2020-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.