http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111171229-B

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filingDate 2020-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2021-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111171229-B
titleOfInvention Preparation method of nucleic acid immobilized microspheres
abstract The invention provides a preparation method of nucleic acid immobilized microspheres, which can effectively immobilize nucleic acid molecules. The method comprises the following steps of dissolving water-soluble functional acrylamide azide monomer, acrylamide monomer, N-methylene-bisacrylamide and polyvinylpyrrolidone in a mixed solvent of isopropanol and water; adding a free radical initiator, heating to 70-90 ℃ from room temperature, and carrying out polymerization reaction to obtain the target functional polymer microspheres; the polymer microsphere can effectively realize the immobilization of nucleic acid on the microsphere through the reaction of the reactive azide group and the alkynyl nucleic acid molecule.
priorityDate 2020-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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