http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111154746-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y305-01004 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
filingDate | 2020-01-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-111154746-B |
titleOfInvention | Amidase mutant and application thereof in catalytic synthesis of 2-chloronicotinic acid |
abstract | The invention discloses an amidase mutant and application thereof in catalytic synthesis of 2-chloronicotinic acid, belonging to the technical field of enzyme engineering. The amidase mutant is obtained by carrying out single-site mutation or multi-site mutation on 378 th position, 402 th position or 403 th position of an amino acid sequence shown in SEQ ID NO. 1. Compared with parent strain, the amidase mutant provided by the invention has greatly improved activity, and the activity of the reaction enzyme is still kept in a higher state when a crude extract of the amidase or whole cells of engineering bacteria are used for catalysis. In addition, the amidase mutant provided by the invention can adapt to the catalytic temperature of 30-55 ℃, and lays a foundation for the industrial enzymatic production of 2-chloronicotinic acid. |
priorityDate | 2020-01-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 312.