http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111154734-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e7e4140486ab9e1d16b147f60ac54835 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 |
| filingDate | 2019-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_53a49b259e025812984c6abb8be306f2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6af4679a71bac9258ef18d9d1c79e9be http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ccfc78812a3fed6fba5e30dc24bbeb58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_339ac2f9d4efcba7383404920f188bb5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_92b6510cd4708c614f0cd6d4bc675ff2 |
| publicationDate | 2020-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111154734-A |
| titleOfInvention | A kind of preparation method of Antarctic krill thrombolytic enzyme |
| abstract | The invention discloses an Antarctic krill thrombolytic enzyme and a preparation method thereof. It comprises the following steps: 1) crushing Antarctic krill at low temperature; 2) mixing with pre-cooled 0.1-0.3mol/L, pH 6-8 phosphate buffer, and centrifuging; 3) supernatant and 20-75% (w /v) Ammonium sulfate was mixed, precipitated overnight at 4°C, centrifuged, and the precipitate was dissolved in a pH 7.0-7.5 Tris-HCl buffer containing 2-10mM CaCl 2 , dialyzed and desalted at 4°C for 12-36h, the dialysate was freeze-concentrated, and vacuum freeze-dried 4) Then adopt Sepharose F ion exchange column chromatography, 40-85% (w/v) ammonium sulfate 4 ℃ of overnight precipitation, dialysis desalting, dialysate freeze concentration, vacuum freeze-drying; 5) adopt Sephadex G-75 coagulation again Gel filtration chromatography, 60-95% (w/v) ammonium sulfate overnight precipitation, dialysis desalination, freeze-concentration of the dialysate, and vacuum freeze-drying to obtain Antarctic krill thrombolytic enzyme. The separation efficiency of the target product of the invention is significantly improved, the yield is high, the efficient separation of Antarctic krill enzymes is realized, and the added value of Antarctic krill products is significantly increased. |
| priorityDate | 2019-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 76.