| abstract |
The invention provides a cell culture medium, a preparation method and application thereof, and relates to the technical field of cell culture. Wherein, the basic culture medium can effectively keep the cell environment stable and simultaneously keep the activity of the cells. By adding the cell factor, the hormone, the protein, the biogenic amine, the inhibitor and the lysin into the basic culture medium, on one hand, animal-derived components in the traditional culture medium can be replaced through the synergistic effect among the components, the stability of the cell culture medium is ensured, and the risk of disease transmission and immune reaction is reduced. On the other hand, the components are matched with each other, so that the activity of the cells can be maintained, the cells can be stably and quickly proliferated, the primary separation efficiency of the cells is greatly improved, and the self-renewal capacity and the tissue repair capacity of the cells are improved. |