| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_47d0c46a02d59ba47699c84801c25a1f |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y201-01
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1007 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-145 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 |
| filingDate |
2020-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1a76a19f9bad26dd27b66c512e0a588c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d6c654cd2e18d15b4a2e0921f7f61b46
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da52b2a90557466dcf2d876dc936660b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e9793d61b84c56b9fad36e58941c78e2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5448e7322de09095b8a69d1aa8126622
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e9bc543a009c60c3513cc0ed667847f6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f14beee9a8d213429c6d1c956705b249
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4af46ad20ef2ac9ac35e9e1d1d184fdc |
| publicationDate |
2020-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-111154683-A |
| titleOfInvention |
A kind of optimized culture method of methylbutyric acid bacteria and its application |
| abstract |
The invention discloses an optimized culture method and application of methylbutyric acid bacteria. It uses type 1 methylase to methylate the exogenous plasmid in the TOP10 strain to obtain the methylated plasmid DNA. The methods employed include: (1) screening of media suitable for Clostridium; (2) screening for replicons that exist stably in Clostridium; (3) determining the optimal methylase; under all optimal conditions, The methylated plasmid DNA can be transferred into the methylated butyric acid bacteria with the restriction modification system, without being sheared and degraded, and the subsequent metabolic pathway modification can be carried out. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113106047-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112760256-A |
| priorityDate |
2020-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |