http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111153999-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-73 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-76 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-22 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1037 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2818 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2803 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-395 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P35-00 |
| filingDate | 2020-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111153999-B |
| titleOfInvention | CTLA-4 nanobody, preparation method and application thereof |
| abstract | The invention belongs to the technical field of biomedicine, and particularly relates to a CTLA-4 nanobody, a preparation method and application thereof. The use of nanobodies can improve or synergistically enhance the binding ability to antigens. Nanobodies do not have a complete antibody structure, lack Fc end and Y-shaped structure, and are not easily recognized after antigens are combined with nanobodies, which can easily escape the capture of the immune system. The technical means mainly include: connecting the antigen with the carrier; transferring it into competent cells and extracting the recombinant plasmid; then transfecting it into the host bacteria for expression; using the antigen protein to immunize camelids; collecting peripheral blood and extracting lymphocyte samples The total RNA was reverse transcribed into cDNA, amplified to obtain VHH library fragments, the VHH library fragments were inserted into the expression vector, and transformed into competent cells to construct an antibody immune library; screen the nanobodies in the antibody immune library; Nanobodies bind to CTLA-4 antigen. |
| priorityDate | 2019-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 240.