http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111139228-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y205-01019 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1092 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8275 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 2019-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111139228-B |
| titleOfInvention | Glyphosate-resistant plant EPSPS enzyme double-mutant and cloning, expression and application thereof |
| abstract | The invention belongs to the technical field of genetic engineering, and particularly relates to a method for improving glyphosate resistance of brassica napus by directional evolution of EPSPS enzyme derived from brassica napus through a gene mutation technology, and discloses an application of a mutant and a coding gene thereof in the field of cultivation of novel glyphosate-resistant crops. The glyphosate-resistant plant EPSPS enzyme double mutant provided by the invention is obtained by modifying a cabbage type rape EPSPS gene BnEPSPS through an in vitro directed evolution technology, so that the glyphosate resistance is improved, and further, plant-derived EPSPS double mutants BnEV1 and BnEV2 with glyphosate resistance are obtained. Enzymatic property determination of the mutants BnEV1 and BnEV2 shows that the glyphosate tolerance is obviously improved, the application of the mutants to plants is verified to improve the plant resistance, important genetic resources can be provided for cultivating new varieties of transgenic glyphosate-resistant rapes which are more acceptable to consumers, and the mutants have wide application value. |
| priorityDate | 2019-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 822.