http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111139216-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_31b3eadca71363bf61f93cd511e3e84d |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M33-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M41-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-07 |
| filingDate | 2019-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_278a412753af917aeaf473c277d7ba75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2063cc68e851369dca7e2436c7879fa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_080368718111fe17290205247ed7ba53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc9b32c2ff05e33a9fb1e7013ecaef4f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_11ad7932a8ebf2a83ba752fa61f7847a |
| publicationDate | 2020-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111139216-A |
| titleOfInvention | High-throughput, automated cell continuous culture method and uses thereof |
| abstract | The invention discloses a high-throughput and automatic continuous cell culture method and application thereof. The method comprises the following steps: (1) providing a cell culture, inoculating and culturing; (2) settling the cells, and removing the supernatant in the reactor; (3) adding a fresh culture medium; (4) calculating a discharge rate (B) based on the cell density in the supernatant, maintaining a target cell density; (5) repeating the steps (2) to (4) until the continuous culture of the cells is completed. Compared with the traditional perfusion reactor, the cell continuous culture model provided by the invention has the advantages of large flux, high automation degree, small culture volume and low cost; compared with the culture by using a shake tube, the reactor has strong characteristics, high representativeness of the culture result, difficult occurrence of human errors and high reliability. In addition, the invention does not need to use an additional cell interception device in the perfusion reactor, thereby solving the problem that the cell interception device is easy to block. |
| priorityDate | 2018-11-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.